If you find the need to utilize any of these lab instruments, please feel free to reach out to Dr. Sobol (robert_sobol@brown.edu) or our lab manager, Dan Ivanov (daniel_ivanov@brown.edu).

We are delighted to collaborate with you and assist in advancing your scientific pursuits.

If you find the need to utilize any of these lab instruments, please feel free to reach out to Dr. Sobol (robert_sobol@brown.edu) or our lab manager, Dan Ivanov (daniel_ivanov@brown.edu).

We are delighted to collaborate with you and assist in advancing your scientific pursuits.

Nikon Ti2-E inverted confocal microscope with Ax-R

Equipped with 20x, 40x, 60x and Super Fluor 40x Oil Immersion objectives, Fluorescence Widefield Illumination, Ax-R 2k Resonant + Galvo Scan Head, 355 microscan laser with external 405/473 lines, NIS Elements Software, a Black Enclosure for light sensitive applications and a temperature control unit and gas chamber for use with Cage and Stage Top Incubators.

Celigo® S Imaging Cytometer

Brightfield & Fluorescence Installed with Classic Collection Application Suite Proprietary large-field F-theta optics with superior edge contrast Galvanometric mirrors for imaging of large areas LED-based brightfield imaging with uniform illumination LED-based fluorescence imaging (4 channels, Blue, Green, Red & Far Red) 4 Mega-pixel CCD camera Magnification (3.5x, resolution 1mm/pixel to 8mm/pixel; 0.25NA) Rapid LED-based hardware auto-focus Computer System.

FiberComb® Molecular Combing System (Genomic Vision)

This system is for the analysis of single DNA molecules to study real-time dynamics of genome replication, analysis of replication defects and the impact of genotoxins on replication.

NanoDrop 2000

Spectrophotometer for DNA, RNA, and protein quantification.

PTC Tempo Deepwell 96-well Thermal Cycler (Bio-Rad)

PCR thermocycler for 10–125 μl recommended reaction volumes; Maximum ramp rate, 2.5°C/sec; Average ramp rate, 2.0°C/sec; Temperature accuracy, ±0.2°C of programmed target at 90°C; Temperature uniformity, ±0.4°C well-to-well within 10 sec of arrival at 90°C.

Nikon Ti2-E inverted confocal microscope with Ax-R

Equipped with 20x, 40x, 60x and Super Fluor 40x Oil Immersion objectives, Fluorescence Widefield Illumination, Ax-R 2k Resonant + Galvo Scan Head, 355 microscan laser with external 405/473 lines, NIS Elements Software, a Black Enclosure for light sensitive applications and a temperature control unit and gas chamber for use with Cage and Stage Top Incubators.

The 5425 R with RotorFA-24×2 is a refrigerated 24-place microcentrifuge for a broad range of molecular and cell biology applications using tubes from 0.2 to 5 mL. The refrigerated Centrifuge 5425 R provides precise temperature control for processing temperature-sensitive samples with its advanced cooling system.

Brightfield & Fluorescence Installed with Classic Collection Application Suite Proprietary large-field F-theta optics with superior edge contrast Galvanometric mirrors for imaging of large areas LED-based brightfield imaging with uniform illumination LED-based fluorescence imaging (4 channels, Blue, Green, Red & Far Red) 4 Mega-pixel CCD camera Magnification (3.5x, resolution 1mm/pixel to 8mm/pixel; 0.25NA) Rapid LED-based hardware auto-focus Computer System.

For analysis of cells in our tissue culture room. Includes a DMIL LED with TL illumination column, with integrated HC Optics and LED Illumination for transmitted light (> 50.000 h life time) with automatic brightness adjustment to contrast method and auto on/off function including integrated Fluorescence axis, manual shutter and with 3-fold filter slider. 20 mm field of view, high stability and large working distances provide optimum microscopy conditions. Also equipped with a K3M camera with a 3072 px x 2048 px sensor at 21 frames per second.

This system is for the analysis of single DNA molecules to study real-time dynamics of genome replication, analysis of replication defects and the impact of genotoxins on replication.

This system is for the analysis of single DNA molecules to study real-time dynamics of genome replication, analysis of replication defects and the impact of genotoxins on replication.

Spectrophotometer for DNA, RNA, and protein quantification.

The ChemiDoc MP Imaging System is a full-feature instrument for imaging and quantifying nucleic acid and protein in gels and western blots. It is capable of imaging fluorescent western blots (RGB, far-red, near-IR), chemiluminescent western blots, all common nucleic acid and protein gel stains, plus stain-free gels.

PTC Tempo Deepwell 96-well Thermal Cycler (Bio-Rad)

PCR thermocycler for 10–125 μl recommended reaction volumes; Maximum ramp rate, 2.5°C/sec; Average ramp rate, 2.0°C/sec; Temperature accuracy, ±0.2°C of programmed target at 90°C; Temperature uniformity, ±0.4°C well-to-well within 10 sec of arrival at 90°C.