Wong Lab Research

Multicellular tumor invasion in 3D matrix
Multicellular tumor invasion and the extracellular matrix. Epithelial tissues are enclosed by basement membrane. During tumor invasion and EMT, tissues disorganize and disseminate into the surrounding interstitial matrix. Tumor cells can further deposit and reorganize the matrix into a more fibrotic architecture. After Leggett et al. Biomaterials Science (2017)

The dissemination of tumor cells to distant organs during metastasis is responsible for over 90% of cancer-related fatalities. We are interested in how cancer cells transition between individual and collective migration in a microenvironmental context. As a case study, we investigate the epithelial-mesenchymal transition (EMT), classically associated with developmental pathways that weaken cell-cell adhesion and upregulate a pro-migratory phenotype with vimentin intermediate filaments. We utilize quantitative and physical methods that include biomaterials engineering, live cell imaging, traction force microscopy, computer vision and topology-based machine learning.

mechanical patterning and 3D printing
Creation methods of higher-dimensional 2D materials and the multifunctionality of resulting surface patterns. Adapted from Chen et al. Advanced Materials (2017)

The creation of three-dimensional (3D) structures from two-dimensional (2D) nanomaterial building blocks enables novel chemical, mechanical or physical functionalities that cannot be realized with planar thin films or in bulk materials. We are interested in using mechanical deformations to encode complex topographical patterns in 2D nanomaterials (e.g. graphene). We also utilize light-directed 3D printing to fabricate hydrogels via ionic and covalent crosslinking, enabling new microfluidic and shape-morphing devices. We also have extensive expertise with conventional soft and nano lithography techniques.