Methodology

• Confocal Laser-Scanning microscopy and conjugated fluorescent antibodies.
  • Visualize and quantify microbial adherence directly on orthopaedic explants.
  • Optical Sectioning of Topographically Complex Samples
  • Electronic Reconstruction

 

Design of FCab and Confocal Microscopy

• Gram Positive
• Fluorescein Isothiocyanate (FITC)
• Ex 492nm-Em 518nm (Green)
• Gram Negative
• Alexa Fluor 594
• Ex 590nm-Em 617nm (Red)
• Eukaryotic Tissue
• 4’, 6-Diamidino-2-Phenindole, Dihydrochloride (DAPI)
• Binds to AT-rich regions of DNA (Nuclear stain)
• Ex 358nm-Em 461nm (Blue)