Google Scholar Citations Link – for a full listing of papers and published abstracts.

*Bolded names indicate graduate or undergraduate students in the Darling Lab.


Dubay R, Darling EM, Fiering J. (2023) Microparticles with tunable, cell-like properties for quantitative acoustic mechanophenotyping. Microsyst Nanoeng. PMCID: PMC10336031. DOI: 10.1038/s41378-023-00556-6 (open access)

Tandar CE, Dubay R, Turo MJ, Doyle RP, Darling EM, Fiering J. (2023) Physiologically relevant microparticles with mechanically tunable properties for acoustophoretic and microfluidic device calibration. Proc Mtgs Acoust. 50, 045004 (2022). DOI: 10.1121/2.0001809


Gutierrez RA, Fonseca VC, Darling EM. (2022) Chondrogenesis of Adipose-Derived Stem Cells Using an Arrayed Spheroid Format. Cell Mol Bioeng. PMCID: PMC9751248. DOI: 10.1007/s12195-022-00746-8 (open access viewing)

Dempsey ME, Chickering GR, González-Cruz RD, Fonseca VC, Darling EM. (2022) Discovery of surface biomarkers for cell mechanophenotype via an intracellular protein-based enrichment strategy. Cell Mol Life Sci. 79(320). PMCID: PMC9239330. DOI: 10.1007/s00018-022-04351-w

Stathatou P, Athanasiou C, Tsezos M, Goss J, Blackburn C, Tourlomousis F, Mershin A, Sheldon BW, Padture NP, Darling EM, Gao H, Gershenfeld N. (2022) Lead removal at trace concentrations from water by inactive yeast cells. Nature Comm Earth Environ. DOI: 10.1038/s43247-022-00463-0 (open access)

2021Glowing dish

Dempsey ME, Woodford-Berry O, Darling EM. (2021) Quantification of antibody persistence for cell surface protein labeling. Cell Mol Bioeng. 14: 267-277. PMCID: PMC8175540. DOI: 10.1007/s12195-021-00670-3

Dubay R, Urban JN, Darling EM. (2021) Single‐Cell Microgels for Diagnostics and Therapeutics. Adv Func Mat. PMCID: PMC9629779. DOI: 10.1016/j.biomaterials.2021.120684

Gutierrez RA, Fang W, Kesari H*, Darling EM*. (2021) Force sensors for measuring microenvironmental forces during mesenchymal condensation. Biomaterials. 270: 120684. PMCID: PMC7906959. DOI: 10.1016/j.biomaterials.2021.120684 (*Co-corresponding author)

Parsons AM, Darling EM. (2021) Temporal responsiveness of adipose-derived stem/stromal cell immune plasticity. Exp Cell Rep. 406(1): 112738. PMID: 34270981. DOI: 10.1016/j.yexcr.2021.112738


Dubay R, Fiering J, Darling EM. (2020) Effect of elastic modulus on inertial displacement of cell-like particles in microchannels. Biomicrofluidics. 14(4): 044110. PMCID: PMC7402708. DOI: 10.1063/5.0017770

Sarnik SA, Sutermaster BA, Darling EM. (2020) Mass-Added Density Modulation for Sorting Cells Based on Differential Surface Protein Levels. Cytometry Part A. PMCID: PMC7855113. DOI: 10.1002/cyto.a.24192

Sadick JS, Crawford LA, Cramer HC, Franck C, Liddelow SA, Darling EM. (2020) Generating Cell Type-Specific Protein Signatures from Non-symptomatic and Diseased Tissues. Annals of Biomedical Engineering. 4(8): 2218-2232. PMCID: PMC7416432. DOI: 10.1007/s10439-020-02507-y

2019AFM experiment

Meyer K, Labriola NR, Darling EM, Kaehr BJ. (2019) Shape preserved transformation of biological cells into synthetic hydrogel microparticles. Adv Biosystems. 3(4): 1800285. PMCID: PMC7747388. DOI: 10.1002/adbi.201800285

Sutermaster BA, Darling EM. (2019) Considerations for high-yield, high-throughput cell enrichment: fluorescence versus magnetic sorting. Sci Rep. 9: 227 (2019). PMCID: PMC6338736. DOI: 10.1038/s41598-018-36698-1

Shah MK, Leary EA, Darling EM. (2019) Integration of hyper-compliant microparticles into a 3D melanoma tumor model. J Biomech. 82(3): 46-53. PMCID: PMC6310620. DOI: 10.1016/j.jbiomech.2018.10.018


González-Cruz RD, Dahl KN, Darling EM. (2018) The Emerging Role of Lamin C as an Important LMNA Isoform in Mechanophenotype. Frontiers Cell Devel Biol. 6: 151. PMCID: PMC6224339. DOI: 10.3389/fcell.2018.00151

Azagury A, Fonseca VC, Cho DY, Perez-Rogers J, Baker CM, Steranka E, Goldenshtein V, Calvao D, Darling EM, Mathiowitz E. (2018) Single Step Double-walled Nanoencapsulation (SSDN). J Control Release. 280: 11-19. PMCID: PMC5993621. DOI: 10.1016/j.jconrel.2018.04.048

Labriola NR, Sadick JS, Morgan JR, Mathiowitz E, Darling EM. (2018) Cell mimicking microparticles influence the organization, growth, and mechanophenotype of stem cell spheroids. Ann Biomed Eng. 46(8): 1146-1159. PMCID: PMC6039261. DOI: 10.1007/s10439-018-2028-4

Parsons AM, Ciombor M, Liu P, Darling EM. (2018) Regenerative potential and inflammation-induced secretion profile of human adipose-derived stromal vascular cells are influenced by donor variability and prior breast cancer diagnosis. Stem Cell Rev Rep. 14(4): 546–557. PMCID: PMC6014910. DOI: 10.1007/s12015-018-9813-1

González-Cruz RD, Sadick JS, Fonseca VC, Darling EM. (2018) Nuclear lamin protein C is linked to lineage-specific, whole-cell mechanical properties. Cell Mol Bioeng. 11(2): 131-142. PMCID: PMC5943047. DOI: 10.1007/s12195-018-0518-y

Dempsey ME*, Marble HD*, Shin T, Fawzi NL, Darling EM. (2018) Synthesis and characterization of a magnetically active 19F molecular beacon. ACS Bioconjug Chem. 29(2): 335-342. PMCID: PMC5821531. DOI: 10.1021/acs.bioconjchem.7b00671 (*Co-first authors)

Labriola NR, Azagury A, Gutierrez R, Mathiowitz E, Darling EM. (2018) Concise Review: Fabrication, customization, and application of cell mimicking microparticles in stem cell science. Stem Cell Trans Med. (7): 232-240. PMCID: PMC5788880. DOI: 10.1002/sctm.17-0207

Clyne AM, Marcolongo M, Darling E, Chahine N. (2018) Translating Mechanobiology to the Clinic: a panel discussion from the 2018 CMBE Conference. Cell Mol Bioeng. 11(6): 531-535. PMCID: PMC6516757. DOI: 10.1007/s12195-018-0556-5


Athanasiou KA, Darling EM, Duraine GD, Hu JC, Reddi AH. (2017) Articular Cartilage, Second Edition. DOI: 10.1201/9781315194158

Labriola NR, Mathiowitz E, Darling EM. (2017) Fabricating polyacrylamide microbeads by inverse emulsification to mimic the size and elasticity of living cells. Biomater Sci. 5: 41-45. PMCID: PMC5201106. DOI: 10.1039/c6bm00692b

Shah MK, Garcia-Pak IH, Darling EM. (2017) Influence of inherent mechanophenotype on competitive cellular adherence. Ann Biomed Eng. 45(8): 2036-2047. PMCID: PMC5529242. DOI: 10.1007/s10439-017-1841-5

Yang X, Darling EM, Herzog W. (2017) Functional properties of chondrocytes and articular cartilage using optical imaging to ccanning probe microscopy. J Orthop Res. PMCID: PMC5839958. DOI: 10.1002/jor.23757

Sadick JS, Darling EM. (2017) Processing fixed and stored adipose-derived stem cells for quantitative protein array assays. Biotechniques. 63(6): 275-280. PMCID: PMC5731247. DOI: 10.2144/000114620

Morss Clyne A, Darling EM, Chahine NO. (2017) Discovering the Keys: Transformative and Translational Mechanobiology. Cell Molec Bioeng. 10(4): 273-274. PMCID: PMC6816652. DOI: 10.1007/s12195-017-0496-5


Sadick JS, Boutin ME, Hoffman-Kim D, Darling EM. (2016) Protein characterization of intracellular target-sorted, formalin-fixed cell subpopulations. Sci Rep. 6: 33999. PMCID: PMC5036045. DOI: 10.1038/srep33999

Beane OS, Fonseca VC, Darling LEO, Darling EM. (2016) Disparate response to methotrexate in stem versus non-stem cells. Stem Cell Rev Reports. 12 (3): 340-51. PMCID: PMC4880537. DOI: 10.1007/s12015-016-9645-9

López-Fagundo C, Livi LL, Ramchal T, Darling EM*, Hoffman-Kim D*. (2016) A biomimetic synthetic feeder layer supports the proliferation and self-renewal of mouse embryonic stem cells. Acta Biomater. 39: 55-64. PMCID: PMC4905775. DOI: 10.1016/j.actbio.2016.04.047 (*Co-corresponding author)

Chen Y, Cossman J, Jayasuriya CT, Li X, Guan Y, Fonseca VC, Yang K, Charbonneau C, Yu H, Kanbe K, Ma P, Darling EM, Chen Q. (2016) Deficient mechanical activation of anabolic transcripts and post-traumatic cartilage degeneration in matrilin-1 knockout mice. PLoS ONE. 11(6): E0156676. PMCID: PMC4896629. DOI: 10.1371/journal.pone.0156676


Dingle YL, Chirila AM, Boutin ME, Livi LL, Labriola NR, Jakubek LM, Morgan JR, Darling EM, Kauer JA, Hoffman-Kim D. (2015) Three-Dimensional Neural Spheroid Culture: An In Vitro Model for Cortical Studies. Tissue Eng C. 21 (12): 1274-83. PMCID: PMC4663656. DOI: 10.1089/ten.tec.2015.0135

Darling EM, DiCarlo D. 2015. High-throughput assessment of cellular mechanical properties. Ann Rev Biomed Eng. 17:35-62. PMCID: PMC8204286. DOI: 10.1146/annurev-bioeng-071114-040545

Labriola NR, Darling EM. 2015. Temporal heterogeneity in single-cell gene expression and mechanical properties during adipogenic differentiation. J Biomech. 28(2015): 1058-66. PMCID: PMC4380682. DOI: 10.1016/j.jbiomech.2015.01.033


Marble HD, Sutermaster BA, Kanthilal M, Fonseca VC, Darling EM. (2014). Gene expression-based enrichment of live cells from adipose tissue produces subpopulations with improved osteogenic potential. Stem Cell Res Ther. 5(5):145. PMCID: PMC4619280. DOI: 10.1186/scrt502

Beane OS, Fonseca VC, Cooper LL, Koren G, Darling EM. (2014). Impact of aging on the regenerative preoperties of bone marrow-, muscle-, and adipose-derived mesenchymal stem/stromal cells. PLoS ONE. 9(12): e115963. PMCID: PMC4277426. DOI: 10.1371/journal.pone.0115963

Kanthilal M, Darling EM. (2014). Characterization of mechanical and regenerative properties of human, adipose stromal cells. Cell Mol Bioeng. 7 (4): 585-97. PMCID: PMC4255916. DOI: 10.1007/s12195-014-0350-y

Toyjanova J, Hannen E, Bar-Kochba E, Darling EM, Henann D, Franck C. (2014). 3D viscoelastic traction force microscopy. Soft Matter. 10: 8095-106. PMCID: PMC4176508. DOI: 10.1039/C4SM01271B

Beane OS, Fonseca VC, Darling EM. (2014). Adipose-derived stem cells retain their regenerative potential after methotrexate treatment. Exp Cell Res. 327 (2014): 222-233. PMCID: PMC4164584. DOI: 10.1016/j.yexcr.2014.06.015

Desai HV, Voruganti IS, Jayasuriya C, Chen Q, Darling EM. (2014). Live-cell, temporal gene expression analysis of osteogenic differentiation in adipose-derived stem cells. Tissue Eng Pt A. 20(5-6): 899-907. PMCID: PMC3938923. DOI: 10.1089/ten.TEA.2013.0761


Athanasiou KA, Darling EM, Duraine GD, Hu JC, Reddi AH. (2013). “Articular Cartilage.” Taylor & Francis Group, LLC: Boca Raton, FL. IBSN: 9781439853245

González-Cruz RD, Darling EM. (2013). Adipose-derived stem cell fate is predicted by cellular mechanical properties. Adipocyte. 2 (2): 87-91. PMCID: PMC3661107. DOI: 10.4161/adip.23015

Darling EM, Athanasiou KA. (2013) Bioactive scaffold design for articular cartilage engineering. Biomedical Technology and Devices Handbook. 2nd edition. Ed. by J. Moore and D. Maitland. DOI: 10.1201/b15085-23


Beane OS, Darling EM. (2012). Isolation, Characterization, and Differentiation of Stem Cells for Cartilage Regeneration. Ann Biomed Eng. 40 (10): 2079-97. PMCID: PMC3457050. DOI: 10.1007/s10439-012-0639-8

González-Cruz RD, Fonseca VC, Darling EM. (2012). Cellular mechanical properties reflect differentiation potential of adipose-derived mesenchymal stem cells. Proc Natl Acad Sci USA. 109 (24): E1523-9. PMCID: PMC3386052. DOI: 10.1073/pnas.1120349109

Desai HV, Darling EM. (2012). Force Scanning for Simultaneous Collection of Topographical and Mechanical Properties. Microscopy and Analysis, January 2012. Volume 26, Issue 1. DOI: 10.1002/micro.235/full


Darling EM. (2011). Force scanning: A rapid, high-resolution approach for spatial mechanical property mapping. Nanotechnology. 22 (17) 175707. PMCID: PMC3150532. DOI: 10.1088/0957-4484/22/17/175707 *Recognized by Nanotechnology in their “Highlights 2011” Collection.

Gilchrist CL, Darling EM, Chen J, Setton LA. (2011). Extracellular matrix ligand and stiffness modulate immature nucleus pulposus cell‐cell interactions. PLoS One. 6 (11): e27170. PMCID: PMC3210142. DOI: 10.1371/journal.pone.0027170


Yim EKF, Darling EM, Kulangara K, Guilak F, Leong KW. (2010). Nanotopography-induced changes in focal adhesions, cytoskeletal organization, and mechanical properties of human mesenchymal stem cells. Biomaterials. 31 (2010): 1299-1306. PMCID: PMC2813896. DOI: 10.1016/j.biomaterials.2009.10.037

Darling EM*, Wilusz RE*, Bolognesi MP, Zauscher S, Guilak F. (2010). Spatial mapping of the biomechanical properties of the pericellular matrix of articular cartilage measured in situ via atomic force microscopy. Biophys J. 98: 2848-2856. PMCID: PMC2884253. DOI: 10.1016/j.bpj.2010.03.037 (*co-first author)

2009 (Darling Lab at Brown University)

Darling EM, Pritchett PE, Evans BA, Superfine R, Zauscher S, Guilak F. (2009). Mechanical properties and gene expression of chondrocytes on micropatterned substrates following dedifferentiation in monolayer. Cell Molec Bioeng. 2 (3): 395-404. PMCID: PMC2898162. DOI: 10.1007/s12195-009-0077-3

Athanasiou KA, Darling EM, Hu JCY. (2009). Articular Cartilage Tissue Engineering. Morgan & Claypool Publishers: San Rafael, CA. DOI: 10.2200/S00212ED1V01Y200910TIS003


Darling EM, Guilak F. (2008) A neural network model for cell classification based on single-cell biomechanical properties. Tissue Eng Part A. 14 (9): 1507-1515. PMCID: PMC2748927. DOI: 10.1089/ten.tea.2008.0180

Coles J, Blum J, Jay G, Darling EM, Guilak F, Zauscher S. (2008) In situ friction measurement on murine cartilage by atomic force microscopy. J Biomech. 41 (3): 541-548. PMCID: PMC2274896. DOI: 10.1016/j.jbiomech.2007.10.013

Darling EM, Topel M, Zauscher S, Vail TP, Guilak F. (2008) Viscoelastic properties of human mesenchymally-derived stem cells and primary osteoblasts, chondrocytes, and adipocytes. J Biomech. 41 (2): 454-464. PMCID: PMC2897251. DOI: 10.1016/j.jbiomech.2007.06.019


Darling EM, Zauscher S, Block JA, Guilak F. (2007) A thin-layer model for viscoelastic, stress-relaxation testing of cells using atomic force microscopy: Do cell properties reflect metastatic potential? Biophys J. 92: 1784-1791. PMCID: PMC1796808. DOI: 10.1529/biophysj.106.083097


Darling EM, Zauscher S, Guilak F. (2006) Viscoelastic properties of zonal articular chondrocytes measured by atomic force microscopy. Osteoarthritis Cartilage. 14 (6): 571-579. PMID: 16478668. DOI: 10.1016/j.joca.2005.12.003

2005 (EM Darling at Duke University)

Darling EM, Athanasiou KA. (2005) Growth factor impact on articular chondrocyte subpopulations. Cell Tissue Res. 322: 463-473. PMID: 16047167. DOI: 10.1007/s00441-005-0020-4

Darling EM, Athanasiou KA. (2005) Retaining zonal chondrocyte phenotype by means of novel growth environments. Tissue Eng. 11 (3/4): 395-403. PMID: 15871669. DOI: 10.1089/ten.2005.11.395

Darling EM, Athanasiou KA. (2005) Rapid phenotypic changes in passaged articular chondrocyte subpopulations. J Orthop Res. 23 (2): 425-432. PMID: 15734258. DOI: 10.1016/j.orthres.2004.08.008


Darling EM, Hu JCY, Athanasiou KA. (2004) Zonal and topographical gene expression in articular cartilage. J Orthop Res. 22 (6): 1182-1187. PMID: 15475195. DOI: 10.1016/j.orthres.2004.03.001

Darling EM, Athanasiou KA. (2004) Bioactive scaffold design for articular cartilage engineering. Biomedical Technology and Devices Handbook. Ed. by J. Moore and G. Zouridakis. Chapter 21. CRC Press: Boca Raton, FL. ISBN: 9780849311406

2003 (EM Darling at Rice University)

Darling EM, Athanasiou KA. (2003) Biomechanical strategies for articular cartilage regeneration. Ann Biomed Eng. 31: 1114-1124. PMID: 14582614. DOI: 10.1114/1.1603752

Darling EM, Athanasiou KA. Articular cartilage bioreactors and bioprocesses. (2003) Tissue Eng. 9 (1): 9-26. PMID: 12625950. DOI: 10.1089/107632703762687492