Hyper-compliant microparticles

HCMP in spheroids

We have developed techniques to create polymer particles that replicate the size and stiffness of living cells, which was a previously uncharted region in the large world of microparticle fabrication. Our group is now exploring many applications for these hyper-compliant microparticles (HCMPs) in fields as diverse as device calibration, in situ probes, regenerative medicine, and drug delivery.

  1. Dubary R, Urban JN, Darling EM. (2021) Single‐Cell Microgels for Diagnostics and Therapeutics. Adv Func Mat. PMCID pending. DOI:  10.1016/j.biomaterials.2021.120684
  2. Gutierrez RA, Fang W, Kesari H*, Darling EM*. (2021) Force sensors for measuring microenvironmental forces during mesenchymal condensation. Biomaterials. PMCID: PMC7906959. DOI: 10.1016/j.biomaterials.2021.120684 (*Co-corresponding author)
  3. Dubay R, Fiering J, Darling EM. (2020) Effect of elastic modulus on inertial displacement of cell-like particles in microchannels. Biomicrofluidicsdoi.org/10.1063/5.0017770 
  4. Meyer K, Labriola NR, Darling EM, Kaehr BJ. (2019) Shape preserved transformation of biological cellsMicrofluidic system into synthetic hydrogel microparticles. Adv Biosystems. 3(4): 1800285
  5. Shah MK, Leary EA, Darling EM. (2019) Integration of hyper-compliant microparticles into a 3D melanoma tumor model. J Biomechanics. 82(3): 46-53. PMCID: PMC6310620
  6. Labriola NR, Sadick JS, Morgan JR, Mathiowitz E,Darling EM. (2018) Cell mimicking microparticles influence the
    organization, growth, and mechanophenotype of stem cell spheroids. Ann Biomed Eng. PMCID: PMC6039261 
  7. Labriola NR, Azagury A, Gutierrez R, Mathiowitz E, Darling EM. (2018) Concise Review: Fabrication, customization, and application of cell mimicking microparticles in stem cell science. Stem Cell Trans Med. (7):232-240. PMCID: PMC5788880 
  8. Labriola NR, Mathiowitz E, Darling EM. (2017) Fabricating polyacrylamide microbeads by inverse emulsification to mimic the size and elasticity of living cells. Biomater Sci. 5:41-45. PMCID: PMC5201106