Biomarker discovery and enrichment

Enrichment of cells using molecular beacons

Characterizing single cells allows for the possibility of identifying unique features corresponding to high-value populations. Our group seeks to discover truly novel biomarkers, with a concentration on mesenchymal stem cells. We have explored cellular characteristics spanning mechanophenotype, gene expression, and traditional surface proteins towards the goal of enriching function-specific stem/stromal cell types. These approaches have the potential to generate tailored stem cell populations that will perform a given function with a predictable baseline level, lessening the current problems with unstandardized cell sources used in regenerative medicine therapies.

  1. Sarnik SA, Sutermaster BA, Darling EM. (2020) Mass-Added Density Modulation for Sorting Cells Based on Differential Surface Protein Levels. Cytometry Part A.
  2. Sutermaster BA, Darling EM. (2019) Considerations for high-yield, high-throughput cell enrichment: fluorescence versus magnetic sorting. Sci Rep. 9: 227 (2019). PMCID: PMC6338736.
  3. González-Cruz RD, Sadick JS, Fonseca VC, Darling EM. (2018) Nuclear lamin protein C is linked to lineage-specific, whole-cell mechanical properties. Cell Mol Bioeng. 11(2):131-142. PMCID: PMC5943047
  4. Demspey ME*, Marble HD*, Shin T, Fawzi NL, Darling EM. (2018) Synthesis and characterization of a magnetically active 19F molecular beacon. ACS Bioconjug Chem. 29(2):335-342. PMCID: PMC5821531 *Co-first authors
  5. Sadick JS, Darling EM. (2017) Processing fixed and stored adipose-derived stem cells for quantitative protein array assays. Biotechniques. 63(6): 275-280. PMCID: PMC5731247
  6. Sadick JS, Boutin ME, Hoffman-Kim D, Darling EM. (2016) Protein characterization of intracellular target-sorted, formalin-fixed cell subpopulations. Sci Rep. 6: 33999. PMCID: PMC5036045
  7. Darling EM, Di Carlo D. (2015) High-throughput assessment of cellular mechanical properties. Ann Rev Biomed Eng. 17:35-62. PMID: 26194428
  8. Marble HD, Sutermaster BA, Kanthilal M, Fonseca VC, Darling EM. (2014) Gene expression-based enrichment of live cells from adipose tissue produces subpopulations with improved osteogenic potential. Stem Cell Res Ther. 5 (5):145. PMCID: PMC4619280
  9. Kanthilal M, Darling EM. (2014) Characterization of mechanical and regenerative properties of human, adipose stromal cells. Cell Mol Bioeng. 7 (4): 585-97. PMCID: PMC4255916
  10. Desai HV, Voruganti IS, Jayasuriya C, Chen Q, Darling EM. (2014) Live-cell, temporal gene expression analysis of osteogenic differentiation in adipose-derived stem cells. Tissue Eng Part A. 20(5-6): 899-907. PMCID: PMC3938923
  11. González-Cruz RD, Darling EM. (2013) Adipose-derived stem cell fate is predicted by cellular mechanical properties. Adipocyte. 2 (2): 87-91. PMCID: PMC3661107
  12. González-Cruz RD, Fonseca VC, Darling EM. (2012) Cellular mechanical properties reflect differentiation potential of adipose-derived mesenchymal stem cells. Proc Natl Acad Sci USA. 109 (24): E1523-9. PMCID: PMC3386052
  13. Darling EM, Guilak F. (2008) A neural network model for cell classification based on single-cell biomechanical properties. Tissue Eng Part A. 14 (9): 1507-1515. PMCID: PMC2748927